STRipy is available as both a graphical application and a separate command-line tool for analysing known disease-associated short tandem repeat loci in short-read sequencing data. The graphical version can be used on Linux and macOS systems, requires no bioinformatics knowledge and does not require additional tools to be installed. By selecting a BAM file and a chosen STR locus, the locus can be genotyped on the fly using the free STRipy cloud service.

The "STRipy-pipeline" version is a command-line tool designed for integration into clinical pipelines running on HPC systems. It supports parallel computing and large-scale analysis. STRipy incorporates pathogenic thresholds derived from the literature and population-wide genotyping data, enabling users to easily identify individuals who may be at risk of a repeat expansion disorder.

STRipy's STRs database is a comprehensive, curated online resource for known disease-associated short tandem repeat loci. It was developed as a stand-alone resource separate from the main software and provides information on pathogenicity, locus structure, genomic location and repeat frequencies across populations.

The website also includes online tools to support the interpretation of bioinformatics analysis results. For example, users can annotate STR loci in VCF files on the fly with information about pathogenicity. Additional tools support ExpansionHunter, a widely used STR genotyping tool, by enabling users to generate STR variant catalogues and interpret its raw output. These tools use the STRs database as their underlying information source, including for determining pathogenic repeat thresholds.